EVALUATION OF PRE-PURIFICATION EXTRACTORS OF CATECHIN ISOLATION FROM COMMUNITY GAMBIER (Uncaria Gambiere Roxb)

Sari, Ellyta and Desni Rahman, Elly and Burmawi, Burmawi and Athiya S, Natassya (2023) EVALUATION OF PRE-PURIFICATION EXTRACTORS OF CATECHIN ISOLATION FROM COMMUNITY GAMBIER (Uncaria Gambiere Roxb). AIP Conference Proceedings. ISSN 1757899

	Text 1. EVALUATION OF PRE-PURIFICATION EXTRACTORS OF CATECHIN ISOLATION FROM COMMUNITY GAMBIER.pdf Download (630kB)
	Text 1. Turnitin EVALUATION OF PRE-PURIFICATION EXTRACTORS OF CATECHIN ISOLATION FROM COMMUNITY GAMBIER.pdf Download (1MB)

Abstract

The pre-purification heat extraction process separates the catechins and tannins from the impurities present in the gambier. This process uses water as a solvent, at a ratio of 1: 7 to the sample, with a heating extractor, because at a temperature of 70⁰C, catechins and tannin dissolved in water. The solution is then filtered in a hot state. A cold process further extracted the catechins-tannin extract to separate the catechins and tannins, using a cooling extractor, to obtain sediment. A vacuum filter then filters the deposit to get pre-purification catechins with high levels of catechins. At the same time, the tannin passes from filtration and is accommodated in a tannin reservoir. Pre-purified catechins were dried in an oven at 50⁰C, then weighed and measured their catechins level using a spectrophotometer. This study aims to evaluate the prototype of catechins isolation extract from community gambier (Uncaria Gambier) since this extractor is newly designed and newly operated. The evaluation is done by varying the cooling temperature, the extractor cooling time by 3x washing onto the catechins yield. The highest sediment obtained from the 5⁰C temperature variation and 15 hours cooling time was 817.66 grams of 3 kg of dry gambier, at the highest catechins content of 96%. Thus it is concluded that the best performance of cooling extraction on gambier pre purification is still at 48.55%.

Actions (login required)

View Item